Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Komagataella pastoris |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 18% (w/v) PEG 8000, 0.1 M MES pH 6.0, 0.2 M Ca(OAc)2 | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Komagataella pastoris | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Komagataella pastoris | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Komagataella pastoris X-33 | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Komagataella pastoris | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | Komagataella pastoris X-33 | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | F2QZA4 | - |
- |
Komagataella pastoris X-33 | F2QZA4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating column chromatography, SP Sepharose column chromatography and Superdex S200 gel filtration | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Komagataella pastoris | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Komagataella pastoris X-33 | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Komagataella pastoris | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | - |
Komagataella pastoris X-33 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Komagataella pastoris |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Komagataella pastoris |
SHMT | - |
Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Komagataella pastoris |