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Literature summary for 2.1.2.1 extracted from

  • Zhang, M.; Wu, W.; Chen, Z.
    Structure and function of cytoplasmic serine hydroxymethyltransferase from Pichia pastoris (2018), Biochem. Biophys. Res. Commun., 496, 753-757 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Komagataella pastoris

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 18% (w/v) PEG 8000, 0.1 M MES pH 6.0, 0.2 M Ca(OAc)2 Komagataella pastoris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Komagataella pastoris 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Komagataella pastoris
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Komagataella pastoris X-33
-
tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine Komagataella pastoris
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine Komagataella pastoris X-33
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Komagataella pastoris F2QZA4
-
-
Komagataella pastoris X-33 F2QZA4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating column chromatography, SP Sepharose column chromatography and Superdex S200 gel filtration Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Komagataella pastoris tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Komagataella pastoris X-33 tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine
-
Komagataella pastoris 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine
-
Komagataella pastoris X-33 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Komagataella pastoris

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Komagataella pastoris
SHMT
-
Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Komagataella pastoris