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Literature summary for 2.1.2.1 extracted from

  • Haque, M.; Hirowatari, A.; Nai, N.; Furuya, S.; Yamamoto, K.
    Serine hydroxymethyltransferase from the silkworm Bombyx mori Identification, distribution, and biochemical characterization (2019), Arch. Insect Biochem. Physiol., 102, e21594 .
    View publication on PubMed
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bombyx mori

Protein Variants

Protein Variants Comment Organism
H119A the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
H132A the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
H135A the mutant shows reduced activity compared to the wild type enzyme Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 tetrahydrofolate mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.0031
-
 tetrahydrofolate mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.014
-
 tetrahydrofolate mutant enzyme H132A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.055
-
 tetrahydrofolate wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
1.8
-
 L-serine wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
3.3
-
 L-serine mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
4.2
-
 L-serine mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Bombyx mori
-
 tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O Bombyx mori p50T
-
 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine Bombyx mori
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine Bombyx mori p50T
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-
Bombyx mori p50T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography and Superdex S200 gel filtration Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Bombyx mori
-
hemocyte
-
 Bombyx mori
-
larva
-
 Bombyx mori
-
midgut
-
 Bombyx mori
-
ovary highest expression Bombyx mori
-
silk gland
-
 Bombyx mori
-
testis high expression Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Bombyx mori tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Bombyx mori p50T tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine
-
 Bombyx mori 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine
-
 Bombyx mori p50T 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Bombyx mori

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
 Bombyx mori
SHMT
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bombyx mori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 35 the enzyme shows about 35% activity at 25 or 35°C Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme retains more than 80% of its activity after 30 min at 20-40°C. After 30 min at 45-50°C, the enzyme shows about 30% residual activity. The enzyme shows about 30% activity after 30 min at pH 10-12 Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
2 4 about 60% activity at pH 2.0, 100% activity at pH 3.0, about 20% activity at pH 4.0 Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 7 the enzyme retains more than 50% of its activity after 30 min at pH 2.0-7.0 Bombyx mori

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bombyx mori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00065
-
 L-serine mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.00071
-
 L-serine mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.0022
-
 L-serine wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.011
-
 tetrahydrofolate mutant enzyme H132A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.047
-
 tetrahydrofolate mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.074
-
 tetrahydrofolate mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori
0.081
-
 tetrahydrofolate wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C Bombyx mori