Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
tetrahydrofolate | in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax | |
0.18 | - |
L-serine | in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax | |
47 | - |
D-serine | apparent value, in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Plasmodium vivax |
72400 | - |
gel filtration | Plasmodium vivax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-25 gel filtration | Plasmodium vivax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
crude extract, in 50 mM HEPES (pH 7.0), 1 mM dithiothreitol and 0.5 mM EDTA at 25°C | Plasmodium vivax |
1.86 | - |
after 3.8fold purification, in 50 mM HEPES (pH 7.0), 1 mM dithiothreitol and 0.5 mM EDTA at 25°C | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine + tetrahydrofolate | the binding affinity for D-serine is 150fold lower than that of L-serine | Plasmodium vivax | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | L-serine is the physiological substrate | Plasmodium vivax | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | SHMT also catalyzes the tetrahydrofolate-independent retro-aldol cleavage of 3-hydroxy amino acids | Plasmodium vivax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 49000, SDS-PAGE | Plasmodium vivax |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Plasmodium vivax |
SHMT | - |
Plasmodium vivax |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 30 | the catalytic activity increases upon temperature increment | Plasmodium vivax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
D-serine | in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax | |
0.98 | - |
L-serine | in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax | |
0.98 | - |
tetrahydrofolate | in 50 mM HEPES buffer (pH 7.0), at 25°C | Plasmodium vivax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 50 mM HEPES | Plasmodium vivax |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | - |
Plasmodium vivax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the Kd for binding of the enzyme and pyridoxal 5'-phosphate is 0.00014 mM | Plasmodium vivax |