Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain GS1993 recA- | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L276A | the mutant is in the monomeric state and shows reduced activity | Escherichia coli |
L85A | the apo-L85A mutant enzyme is approximately 75% dimeric and shows reduced activity | Escherichia coli |
L85A/L276A | the mutant is in the monomeric state and shows reduced activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00435 | - |
tetrahydrofolate | apparent value, mutant enzyme L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.00703 | - |
tetrahydrofolate | apparent value, wild type enzyme, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.00716 | - |
tetrahydrofolate | apparent value, mutant enzyme L85A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.0112 | - |
tetrahydrofolate | apparent value, mutant enzyme L85A/L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.14 | - |
L-serine | apparent value, wild type enzyme, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.15 | - |
L-serine | apparent value, mutant enzyme L85A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.2 | - |
L-serine | apparent value, mutant enzyme L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
0.2 | - |
L-serine | apparent value, mutant enzyme L85A/L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
wild type SHMT exists as a dimer in both apo- and holo-forms, ultracentrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A825 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Escherichia coli | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in the 0.0025-0.025 mM subunit concentration range, the wild type SHMT is a dimer, either in the presence or absence of cofactor. The apo-L85A mutant enzyme is approximately 75% dimeric | Escherichia coli |
monomer | the apo-L276A and the apo-L85A mutants are in the monomeric state | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Escherichia coli |
SHMT | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
L-serine | mutant enzyme L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
6.7 | - |
L-serine | mutant enzyme L85A/L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
10.8 | - |
L-serine | mutant enzyme L85A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli | |
11.4 | - |
L-serine | wild type enzyme, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |