Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Carapichea ipecacuanha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
S-adenosyl-L-homocysteine | pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside | Carapichea ipecacuanha | |
0.08 | - |
cephaeline | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.105 | - |
7-O-methyl-N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.107 | - |
N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Carapichea ipecacuanha | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
x * 38700, calculated from sequence | Carapichea ipecacuanha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cephaeline | Carapichea ipecacuanha | the enzyme is involved in emetine biosynthesis. It does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline, the preceding step in the biosynthesis of emetine | S-adenosyl-L-homocysteine + emetine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carapichea ipecacuanha | D3KY98 | i.e. Psychotria ipecacuanha | - |
Purification (Comment) | Organism |
---|---|
N-terminal His-tagged IpeOMT1 enzyme | Carapichea ipecacuanha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Carapichea ipecacuanha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | IpeOMT1 does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline. IpeOMT1 shows broad substrate specificity towards the intermediates in emetine biosynthesis. IpeOMT1 dominantly catalyzes 6-O-methylation of the 6,7-dihydroxy- and 6-hydroxy-7-methoxyisoquinolines. The regional substrate specificity at the last step of emetine biosynthesis is restricted to 6'-O-methylation | Carapichea ipecacuanha | ? | - |
? | |
S-adenosyl-L-methionine + (R)-norprotosinomenine | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-isococlaurine | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-isoorientaline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-norcoclaurine | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (R,S)-protosinomenine | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (S)-4'-O-methyllaudanosoline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (S)-norprotosinomenine | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 7-O-methyl-N-deacetylisoipecoside | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + 6,7-O,O-dimethyl-N-deacetylisoipecoside | - |
? | |
S-adenosyl-L-methionine + cephaeline | the enzyme is involved in emetine biosynthesis. It does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline, the preceding step in the biosynthesis of emetine | Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? | |
S-adenosyl-L-methionine + cephaeline | 6'-O-methylation | Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? | |
S-adenosyl-L-methionine + N-deacetylisoipecoside | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylipecoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38700, calculated from sequence | Carapichea ipecacuanha |
Synonyms | Comment | Organism |
---|---|---|
IpeOMT1 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Carapichea ipecacuanha |
40 | - |
- |
Carapichea ipecacuanha |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
cephaeline | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.0071 | - |
S-adenosyl-L-homocysteine | pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside | Carapichea ipecacuanha | |
0.0113 | - |
N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.0204 | - |
7-O-methyl-N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Carapichea ipecacuanha |
8 | - |
6-methylation of N-deacetylisoipecoside | Carapichea ipecacuanha |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
cephaeline | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.106 | - |
N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha | |
0.169 | - |
S-adenosyl-L-homocysteine | pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside | Carapichea ipecacuanha | |
0.194 | - |
7-O-methyl-N-deacetylisoipecoside | pH 7.5, 30°C | Carapichea ipecacuanha |