BRENDA - Enzyme Database show
show all sequences of 2.1.1.B75

Regiospecific flavonoid 7-O-methylation with Streptomyces avermitilis O-methyltransferase expressed in Escherichia coli

Kim, B.G.; Jung, B.R.; Lee, Y.; Hur, H.G.; Lim, Y.; Ahn, J.H.; J. Agric. Food Chem. 54, 823-828 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a His-tag fusion protein
Streptomyces avermitilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000, SDS-PAGE
Streptomyces avermitilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces avermitilis
-
-
-
Streptomyces avermitilis MA-4680
-
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces avermitilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone
i.e. (2S)-naringenin
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone
i.e. (2S)-sakuranetin
-
-
?
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone
i.e. (2S)-naringenin
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone
i.e. (2S)-sakuranetin
-
-
?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone
i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone
i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone
i.e. kaempferol, active is identical to that with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
-
-
-
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone
i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone
i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
i.e. genkwanin
-
-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
i.e. genkwanin
-
-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 59% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38000, SDS-PAGE
Streptomyces avermitilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a His-tag fusion protein
Streptomyces avermitilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000, SDS-PAGE
Streptomyces avermitilis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone
i.e. (2S)-naringenin
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone
i.e. (2S)-sakuranetin
-
-
?
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone
i.e. (2S)-naringenin
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone
i.e. (2S)-sakuranetin
-
-
?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone
i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone
i.e. quercetin, 119% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone
i.e. kaempferol, active is identical to that with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
-
-
-
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone
i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone
i.e. isorhamnetin, 57% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
-
-
-
-
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
i.e. genkwanin
-
-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
i.e. apigenin, 23.4% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
i.e. genkwanin
-
-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 59% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 126% of the activity with (2S)-naringenin (i.e. (2S)-5,7,4'-trihydroxyflavanone)
674128
Streptomyces avermitilis MA-4680
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38000, SDS-PAGE
Streptomyces avermitilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces avermitilis
Other publictions for EC 2.1.1.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718652
Malla
Production of 7-O-methyl aroma ...
Streptomyces avermitilis
Appl. Environ. Microbiol.
78
684-694
2012
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713037
Joe
Engineering of flavonoid O-met ...
Populus sp.
Mol. Cells
30
137-141
2010
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674128
Kim
Regiospecific flavonoid 7-O-me ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
J. Agric. Food Chem.
54
823-828
2006
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