Literature summary for 2.1.1.B140 extracted from

Liu, Y.; Martinez, A.; Yamashita, S.; Tomita, K.
Crystal structure of human cytoplasmic tRNAHis-specific 5'-monomethylphosphate capping enzyme (2020), Nucleic Acids Res., 48, 1572-1582 .

Search for more literature for 2.1.1.B140

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 200 mM CaCl2	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D110A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
D135A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
D72A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
D72A/G74A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
F136G	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
F40A	inactive	Homo sapiens
G74A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
I111G	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
I166G	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
K201A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
N76A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
Q198A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R118A	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
R204A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R208A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R208A/R209A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R211A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R211A/R212A	inactive	Homo sapiens
R212A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R257A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
R46A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
S165A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
W169F	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
Y37A	the mutant shows severely reduced activity compared to the wild type enzyme	Homo sapiens
Y37F	inactive	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	5 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tRNAHis + S-adenosyl-L-methionine	Homo sapiens	-	methylated tRNAHis + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q7Z5W3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme cannot methylate a tRNAHis mutant with a 5'-triphosphate group at its 5'-end in vitro	Homo sapiens	?	-	-
tRNAHis + S-adenosyl-L-methionine	-	Homo sapiens	methylated tRNAHis + S-adenosyl-L-homocysteine	-	?

Synonyms

Synonyms	Comment	Organism
BCDIN3D	-	Homo sapiens
tRNAHis-specific 5'-monomethylphosphate capping enzyme	-	Homo sapiens