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Literature summary for 2.1.1.B130 extracted from

  • Veiseth, S.V.; Rahman, M.A.; Yap, K.L.; Fischer, A.; Egge-Jacobsen, W.; Reuter, G.; Zhou, M.M.; Aalen, R.B.; Thorstensen, T.
    The SUVR4 histone lysine methyltransferase binds ubiquitin and converts H3K9me1 to H3K9me3 on transposon chromatin in Arabidopsis (2011), PLoS Genet., 7, e1001325 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B130

Activating Compound

Activating Compound Comment Organism Structure
ubiquitin addition of free ubiquitin stimulates enzymatic activity of the SUVR4 protein on histone H3 but does not affect its specificity. In presence of ubiquitin, methylation of H3K9me1 modified peptides increases 2.5-3fold. Trimethylation of the H3K9me2 peptide is observed in the presence of ubiquitin Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information presence of trimethyl-H3K4 reduces SUVR4 activity significantly Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8W595
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in the absence of ubiquitin, SUVR4 converts 40.9% of the H3K9me1 peptide to H3K9me2, while 0% is converted to H3K9me3. In the presence of ubiquitin, 90.2% of the H3K9me1 peptide is converted to H3K9me2 while 3.5% is converted to H3K9me3. When H3K9me2 peptides are used as substrate, there is no conversion to H3K9me3 above background level in the absence of ubiquitin. When ubiquitin is present together with SUVR4, a 16.4% conversion from H3K9me2 to H3K9me3 is found Arabidopsis thaliana ?
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N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine
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 Arabidopsis thaliana N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine
-
 ?
N6-methyl-L-lysyl9-[histone H3] + 2 S-adenosyl-L-methionine
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 Arabidopsis thaliana N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + 2 S-adenosyl-L-homocysteine
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 ?
N6-methyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine
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 Arabidopsis thaliana N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine
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 ?

Synonyms

Synonyms Comment Organism
SUVR4
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 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the N-terminal WIYLD domain of the SUVR4 HKMTase binds ubiquitin and the SUVR4 product specificity shifts from di- to trimethylation in the presence of free ubiquitin, enabling conversion of H3K9me1 to H3K9me3 in vitro. SUVR4 in vivo specifically converts H3K9me1 to H3K9me3 at transposons and pseudogenes and has a locus-specific repressive effect on the expression of such elements. This repression involves both DNA methylation-dependent and -independent mechanisms Arabidopsis thaliana