Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.B120 extracted from

  • Kim, H.J.; McCarthy, R.M.; Ogasawara, Y.; Liu, Y.; Mansoorabadi, S.O.; LeVieux, J.; Liu, H.
    GenK-catalyzed C-6' methylation in the biosynthesis of gentamicin isolation and characterization of a cobalamin-dependent radical SAM enzyme (2013), J. Am. Chem. Soc., 135, 8093-8096 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B120

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Micromonospora echinospora

Organism

Organism UniProt Comment Textmining
Micromonospora echinospora A0A0K0K526
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 1 equivalent each of 5'-deoxyadenosine and S-adenosyl-homocysteine are produced for each methylation reaction catalyzed by GenK. The S-methyl group from S-adenosyl-L-methionine is transferred to cobalamin and the aminoglycoside product during the course of the reaction. H atom abstraction from C6' of GenX2 is stereoselective for the pro-R site Micromonospora echinospora ?
-
-
additional information no substrates: 6'-fluorogentamicin X2, 6'-O-methylgentamicin X2, 5'-fluorogentamicin X2 Micromonospora echinospora ?
-
-
S-adenosyl-L-methionine + gentamicin X2
-
 Micromonospora echinospora S-adenosyl-L-homocysteine + G418
-
 ?

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center enzyme harbors a single [4Fe-4S] cluster per monomer. 3.5 equivalents of iron and 3.8 equivalents of sulfide are bound to each GenK monomer Micromonospora echinospora
Cobalamin
-
 Micromonospora echinospora
methylcobalamin activity is nearly maximized in the presence of 1 mM methylcobalamin Micromonospora echinospora