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Literature summary for 2.1.1.B118 extracted from

  • Zhou, W.; Fisher, P.M.; Vanderloop, B.H.; Shen, Y.; Shi, H.; Maldonado, A.J.; Leaver, D.J.; Nes, W.D.
    A nematode sterol C4alpha-methyltransferase catalyzes a new methylation reaction responsible for sterol diversity (2019), J. Lipid Res., 61, 192-204 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B118

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q9TYP1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 3-keto-enol tautomerism mechanism linked to SAM-dependent methylation. Products show C4alpha configuration. No substrates: cholesterol, lathosterol, cholestanol or zymosterol, cholest-1-ene-3-one, cholest-4-ene-3-one and cholest-4-enol Caenorhabditis elegans ?
-
-
S-adenosyl-L-methionine + cholest-3-one
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + 4alpha-methyl-cholest-3-one
-
 ?
S-adenosyl-L-methionine + cholest-5-en-3-one
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + 4alpha-methyl-cholest-5-en-3-one
-
 ?
S-adenosyl-L-methionine + cholest-7-en-3-one i.e. lathosterone Caenorhabditis elegans S-adenosyl-L-homocysteine + 4alpha-methyl-cholest-7-en-3-one
-
 ?
S-adenosyl-L-methionine + cholestanone
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + 4alpha-methyl-cholestanone
-
 ?

Subunits

Subunits Comment Organism
? x * 37800, calculated from sequence Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
4-SMT
-
 Caenorhabditis elegans
H14E04
-
 Caenorhabditis elegans
SMTR-1
-
 Caenorhabditis elegans

General Information

General Information Comment Organism
physiological function reaction mechanism of C4-methyl sterol formation is a two-step process of bound 3-oxo sterol undergoing active site isomerization to the 4-enol intermediate that is methylated by SAM in a stereoselective manner to yield the C4alpha- (equatorial) methyl group, followed by rearrangement of the enol back to the 3-oxo form Caenorhabditis elegans