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Literature summary for 2.1.1.97 extracted from

  • Fawaz, F.; Jones, G.H.
    Actinomycin synthesis in Streptomyces antibioticus (1988), J. Biol. Chem., 263, 4602-4606.
    View publication on PubMed
Search for more literature for 2.1.1.97

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Streptomyces antibioticus
Na2EDTA
-
 Streptomyces antibioticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 S-adenosyl-L-methionine
-
 Streptomyces antibioticus
0.64
-
 3-Hydroxyanthranilate
-
 Streptomyces antibioticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, SDS-PAGE Streptomyces antibioticus
37000
-
 gel filtration, chromatography in 0.5 M NaCl Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces antibioticus involved in the biosynthesis of the antibiotic actinomycin S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces antibioticus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces antibioticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.052
-
-
 Streptomyces antibioticus

Storage Stability

Storage Stability Organism
-70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3-hydroxyanthranilate involved in the biosynthesis of the antibiotic actinomycin Streptomyces antibioticus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Streptomyces antibioticus