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Literature summary for 2.1.1.95 extracted from
- A picrinine N-methyltransferase belongs to a new family of gamma-tocopherol-like methyltransferases found in medicinal plants that make biologically active monoterpenoid indole alkaloids (2016), Plant Physiol., 170, 1935-1944 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0093 | - |
S-adenosyl-L-methionine | at pH 7.7 and 37°C | Rauvolfia serpentina |  |
| 0.0158 | - |
S-adenosyl-L-methionine | at pH 7.7 and 37°C | Vinca minor | |
| 0.0201 | - |
picrinine | at pH 7.7 and 37°C | Vinca minor | |
| 0.021 | - |
picrinine | at pH 7.7 and 37°C | Rauvolfia serpentina | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | - |
- |
- |
| Vinca minor | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | highest activity in younger leaves | Rauvolfia serpentina | - |
| leaf | highest activity in younger leaves | Vinca minor | - |
| root | - |
Rauvolfia serpentina | - |
| root | - |
Vinca minor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + picrinine | - |
Rauvolfia serpentina | S-adenosyl-L-homocysteine + N-methylpicrinine | - |
? | |
| S-adenosyl-L-methionine + picrinine | - |
Vinca minor | S-adenosyl-L-homocysteine + N-methylpicrinine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, calculated from amino acid sequence | Rauvolfia serpentina |
| ? | x * 35000, calculated from amino acid sequence | Vinca minor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-TLMT | - |
Rauvolfia serpentina |
| gamma-TLMT | - |
Vinca minor |
| gamma-tocopherol-like N-methyltransferase | - |
Rauvolfia serpentina |
| gamma-tocopherol-like N-methyltransferase | - |
Vinca minor |
| PiNMT | - |
Rauvolfia serpentina |
| PiNMT | - |
Vinca minor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
- |
Rauvolfia serpentina |
| 22 | - |
- |
Vinca minor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Vinca minor |
| 7.5 | - |
- |
Rauvolfia serpentina |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vinca minor | calculated from amino acid sequence | - |
5.4 |
| Rauvolfia serpentina | calculated from amino acid sequence | - |
6.9 |
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Release 2023.1 (January 2023)
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