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Literature summary for 2.1.1.91 extracted from

  • Harper, D.B.; Kennedy, J.T.
    Purification and properties of S-adenosylmethionine: aldoxime O-methyltransferase from Pseudomonas sp. N.C.I.B. 11652 (1985), Biochem. J., 226, 147-153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information requires thiol-protecting agents for activity Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 100% inhibition at 1 mM Pseudomonas sp.
Hg2+ 100% inhibition at 0.05 mM Pseudomonas sp.
iodoacetamide 55% inhibition at 0.5 mM Pseudomonas sp.
additional information EDTA and Mg2+ are not inhibitory Pseudomonas sp.
N-ethylmaleimide 56% inhibition at 0.05 mM Pseudomonas sp.
Phenylmercuriacetate 95% inhibition at 0.025 mM Pseudomonas sp.
S-adenosyl-L-homocysteine competitive inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
2-Methylbutyraldoxime
-
Pseudomonas sp.
0.15
-
S-adenosyl-L-methionine
-
Pseudomonas sp.
0.16
-
3-Methylbutyraldoxime
-
Pseudomonas sp.
0.24 0.27 Isobutyraldoxime
-
Pseudomonas sp.
0.67
-
n-Pentanaldoxime
-
Pseudomonas sp.
0.98
-
n-Butyraldoxime
-
Pseudomonas sp.
1.11
-
Methacrylaldoxime
-
Pseudomonas sp.
1.54
-
n-Propionaldoxime
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ has no significant effect Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
2 * 37500, SDS-PAGE Pseudomonas sp.
84000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
N.C.I.B. 11652
-
Pseudomonas sp. NCIB 11652
-
N.C.I.B. 11652
-

Oxidation Stability

Oxidation Stability Organism
labile to O2, thiol-protecting agents are necessary Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
220fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
247.4
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-15°C, 100 mM potassium phosphate buffer, pH 7.5, 5 mM DTT, several months Pseudomonas sp.
0°C, (NH4)2SO4-paste has a half life of 10 weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbutyraldoxime + S-adenosyl-L-methionine highest activity Pseudomonas sp. 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
2-methylbutyraldoxime + S-adenosyl-L-methionine highest activity Pseudomonas sp. NCIB 11652 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
3-methylbutyraldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
3-methylbutyraldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. NCIB 11652 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
isobutyraldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
isobutyraldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. NCIB 11652 isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
methacrylaldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. methacrylaldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
additional information no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime Pseudomonas sp. ?
-
?
additional information no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime Pseudomonas sp. NCIB 11652 ?
-
?
n-butyraldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. n-butyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
n-pentanaldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. n-pentanaldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
n-propionaldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine
-
?
n-propionaldoxime + S-adenosyl-L-methionine
-
Pseudomonas sp. NCIB 11652 n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37500, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
temperature sensitive: 0-3°C are necessary during purification Pseudomonas sp.
35
-
rapid inactivation above Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.25
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
9 10.4 less than 50% of maximal activity below pH 9.0, almost no activity above pH 10.4 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.027
-
S-adenosyl-L-homocysteine
-
Pseudomonas sp.