KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0122 | - |
methylcob(III)alamin | at pH 7.2, temperature not specified in the publication | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 0.1 mM used in assay conditions | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + cob(I)alamin | Methanosarcina acetivorans | methylation of free cob(I)alamin by methylmercaptopropionate, which is the physiologically relevant direction, is observed only in the presence of very high levels of methylmercaptopropionate | methylcob(III)alamin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin resin column chromatography and Superdex S200 gel filtration | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mercaptopropionate + methylcob(III)alamin | - |
Methanosarcina acetivorans | ? | - |
? | |
methanol + cob(I)alamin | methylation of free cob(I)alamin by methylmercaptopropionate, which is the physiologically relevant direction, is observed only in the presence of very high levels of methylmercaptopropionate | Methanosarcina acetivorans | methylcob(III)alamin + H2O | - |
? | |
additional information | coenzyme M does not serve as a methyl acceptor. Methylation of cob(I)alamin with methanol, dimethylsulfide, dimethylamine, and methyl-CoM is not observed, even at high substrate concentrations | Methanosarcina acetivorans | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Cob(I)alamin methyltransferase | - |
Methanosarcina acetivorans |
MMPA:cob(I)alamin methyltransferase | - |
Methanosarcina acetivorans |
MtpA | - |
Methanosarcina acetivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.315 | - |
methylcob(III)alamin | at pH 7.2, temperature not specified in the publication | Methanosarcina acetivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
methylcob(III)alamin | at pH 7.2, temperature not specified in the publication | Methanosarcina acetivorans |