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Literature summary for 2.1.1.90 extracted from

  • Van der Meijden, P.; te Brömmelstroet, B.W.; Poirot, C.M.; van der Drift, C.; Vogels, G.D.
    Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri (1984), J. Bacteriol., 160, 629-635.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.90

Activating Compound

Activating Compound Comment Organism Structure
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri
additional information reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process Methanosarcina barkeri

General Stability

General Stability Organism
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, 90% inhibition Methanosarcina barkeri
Na2SO3 1 mM, 50% inhibition Methanosarcina barkeri
NaNO2 0.1 mM, 90% inhibition Methanosarcina barkeri
NH4Cl 400 mM, 93% inhibition Methanosarcina barkeri
O2 inactivation by O2 and other oxidizing agents Methanosarcina barkeri
pyridoxal 5'-phosphate 1 mM, 74% inhibition Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity Methanosarcina barkeri
Ca2+ metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity Methanosarcina barkeri
Co2+ metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity Methanosarcina barkeri
Mg2+ metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity Methanosarcina barkeri
Mn2+ metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity Methanosarcina barkeri
Ni2+ metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity Methanosarcina barkeri
Sr2+ metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
53000
-
 alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
122000
-
 PAGE with different gel concentrations Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + 5-hydroxybenzimidazolylcobamide Methanosarcina barkeri one of the enzymes responsible for the transmethylation from methanol to coenzyme M Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q46EH3
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive Methanosarcina barkeri

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + 5-hydroxybenzimidazolylcobamide
-
 Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 ?
methanol + 5-hydroxybenzimidazolylcobamide one of the enzymes responsible for the transmethylation from methanol to coenzyme M Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 ?

Subunits

Subunits Comment Organism
trimer alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
5-hydroxybenzimidazolylcobamide 3-4 molecules bound, acting as methyl acceptor Methanosarcina barkeri