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Literature summary for 2.1.1.9 extracted from
- A SABATH Methyltransferase from the moss Physcomitrella patens catalyzes S-methylation of thiols and has a role in detoxification (2012), Phytochemistry, 81, 31-41.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PpSABATH1 full-length cDNA cloned into the pET100/DTOPO vector in Escherichia coli | Physcomitrium patens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM chloride salt, mild inhibitory effect, <25%, PpSABATH1 | Physcomitrium patens |  |
| Cu2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
| Fe2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
| Fe3+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
| Mg2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
| Mn2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
| Zn2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 83.2 | - |
thiobenzoic acid | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens | |
| 94.2 | - |
S-adenosyl-L-methionine | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 5 mM chloride salt, stimulates PpSABATH1 activity by 70% | Physcomitrium patens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physcomitrium patens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant His-tagged PpSABATH1 protein | Physcomitrium patens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0696 | - |
with thiobenzoic acid as substrate, pH 7.5, 25°C | Physcomitrium patens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dithiothreitol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? | |
| furfuryl thiol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? | |
| thiobenzoic acid + S-adenosyl-L-methionine | - |
Physcomitrium patens | methylthiobenzoate + S-adenosyl-L-homocysteine + H+ | - |
? | |
| thiophenol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PpSABATH1 | - |
Physcomitrium patens |
| SABATH methyltransferase | - |
Physcomitrium patens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
loss of 20% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
| 20 | - |
loss of 57% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
| 30 | - |
loss of 64% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
| 50 | - |
loss of 100% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
thiobenzoic acid | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
PpSABATH1 | Physcomitrium patens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
70% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
| 8 | - |
54% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
| 9 | - |
33% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Physcomitrium patens | 2 h after treatment with 250 microM and 500 microM thiobenzoic acid expression of PpSABATH1 is down-regulated | down |
| Physcomitrium patens | 24 h after treatment, expression of PpSABATH1 is up-regulated by eight-fold in plants treated with 250 microM thiobenzoic acid and 16-fold in plants treated with 500 microM thiobenzoic acid | up |
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