BRENDA - Enzyme Database
show all sequences of 2.1.1.9

Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis

Ohsawa, N.; Tsujita, M.; Morikawa, S.; Itoh, N.; Biosci. Biotechnol. Biochem. 65, 2397-2404 (2001)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pavlova pinguis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the bifunctional enzyme also catalyzes the methylation of iodide and to a lesser extent bromide and chloride
696802
Pavlova pinguis
?
-
-
-
-
S-adenosyl-L-methionine + HS-
the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + CH3SH
-
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the bifunctional enzyme also catalyzes the methylation of iodide and to a lesser extent bromide and chloride
696802
Pavlova pinguis
?
-
-
-
-
S-adenosyl-L-methionine + HS-
the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + CH3SH
-
-
-
?
Other publictions for EC 2.1.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733711
Liu
Human liver cytochrome P450 en ...
Homo sapiens
Drug Metab. Dispos.
43
1632-1641
2015
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720650
Takekawa
-
Rice OsHOL1 and OsHOL2 protein ...
Oryza sativa
Plant Biotechnol.
29
103-108
2012
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721115
Obach
Reduction and methylation of z ...
Homo sapiens
Xenobiotica
42
1049-1057
2012
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723371
Zhao
A SABATH Methyltransferase fro ...
Physcomitrella patens
Phytochemistry
81
31-41
2012
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720635
Toda
Isolation and characterization ...
Phaeodactylum tricornutum, Phaeodactylum tricornutum CCAP 1055/1
Phytochemistry
72
337-343
2011
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718580
Schmidberger
Halomethane biosynthesis: Stru ...
Arabidopsis thaliana
Angew. Chem. Int. Ed. Engl.
49
3646-3648
2010
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696997
Itoh
Involvement of S-adenosylmethi ...
Raphanus sativus
BMC Plant Biol.
9
116
2009
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1
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7
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12
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698916
Nagatoshi Y
Arabidopsis HARMLESS TO OZONE ...
Arabidopsis thaliana
J. Biol. Chem.
284
19301-19309
2009
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676510
Coiner
Methylation of sulfhydryl grou ...
Catharanthus roseus
Plant J.
46
193-205
2006
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1
1
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3
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660223
Kocsis
Insertional inactivation of th ...
Arabidopsis thaliana, Zea mays
Plant Physiol.
131
1808-1815
2003
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2
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441597
Attieh
Cloning and functional express ...
Brassica oleracea
Plant Mol. Biol.
50
511-521
2002
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1
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6
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441356
Kim
Arylamine N-methyltransferase ...
Rattus norvegicus
Exp. Mol. Med.
33
23-28
2001
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1
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696802
Ohsawa
Purification and characterizat ...
Pavlova pinguis
Biosci. Biotechnol. Biochem.
65
2397-2404
2001
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441594
Attieh
Purification and properties of ...
Brassica oleracea
Arch. Biochem. Biophys.
380
257-266
2000
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3
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6
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4
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1
2
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441596
Roediger
Thiol methyltransferase activi ...
Homo sapiens
Gut
47
206-210
2000
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1
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700704
Saini
-
Biosynthesis of halomethanes a ...
Brassica oleracea
Plant Cell Environ.
18
1027-1033
1995
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441589
Glauser
Human hepatic microsomal thiol ...
Homo sapiens
Drug Metab. Dispos.
20
247-255
1992
-
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1
1
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441590
Otterness
Mouse liver thiol methyltransf ...
Mus musculus
Drug Metab. Dispos.
14
680-688
1986
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1
1
1
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5
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441591
Drotar
-
Methylation of xenobiotic thio ...
Euglena gracilis
Plant Cell Physiol.
26
847-854
1985
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3
1
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441587
Weisiger
Thiol S-methyltransferase ...
Rattus norvegicus
Methods Enzymol.
77
257-262
1981
-
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2
16
2
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1
1
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4
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15
1
1
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2
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15
1
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441588
Holloway
Gas chromatographic method for ...
Oryctolagus cuniculus
J. Chromatogr.
202
423-429
1980
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441592
Weisiger
Thiol S-methyltransferase: Sug ...
Rattus norvegicus
Biochem. Pharmacol.
29
2885-2887
1980
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3
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441585
Weisiger
Thiol S-methyltransferase from ...
Rattus norvegicus
Arch. Biochem. Biophys.
196
631-637
1979
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-
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1
1
16
-
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2
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2
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1
1
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2
1
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19
1
1
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1
1
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1
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1
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16
-
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2
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1
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2
1
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19
1
1
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1
1
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441593
Weinshilboum
Human erythrocyte thiol methyl ...
Homo sapiens
Clin. Chim. Acta
97
59-71
1979
-
-
-
-
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-
1
1
2
1
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2
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2
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2
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1
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2
1
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2
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2
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1
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441584
Borchardt
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
522
340-353
1978
-
-
-
-
-
-
6
8
1
1
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2
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1
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2
-
1
11
-
1
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1
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1
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6
-
8
1
1
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1
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2
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1
11
-
1
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1
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1
-
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441586
Bremer
-
Enzymic methylation of foreign ...
Bos taurus, Cavia porcellus, Gallus gallus, Mus musculus, Oryctolagus cuniculus, Ovis aries, Rattus norvegicus
Biochim. Biophys. Acta
46
217-224
1961
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7
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7
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7
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17
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7
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
7
-
-
17
-
7
-
-
-
1
-
-
-
-
-
-
-
-
-