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show all sequences of 2.1.1.86

Cloning, sequencing and expression of the genes encoding the sodium translocating N5-methyltetrahydromethanopterin: coenzyme M methyltransferase of the methylotrophic archaeon Methanosarcina mazei G1

Lienard, T.; Gottschalk, G.; FEBS Lett. 425, 204-208 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
all of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea
Methanosarcina mazei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated enzyme
Methanosarcina mazei
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina mazei
O59640 and P80655 and O59638 and P80653 and P80651 and P80654 and P80656 and P80650
O59640: subunit A, P80655: subunit B, O59638: subunit C, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-
Methanosarcina mazei DSM 3647
O59640 and P80655 and O59638 and P80653 and P80651 and P80654 and P80656 and P80650
O59640: subunit A, P80655: subunit B, O59638: subunit C, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
the enzyme uses a transmethylation reaction to drive an energy-conserving sodium ion pump
727499
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
the enzyme uses a transmethylation reaction to drive an energy-conserving sodium ion pump
727499
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
all of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea
Methanosarcina mazei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated enzyme
Methanosarcina mazei
16020
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
the enzyme uses a transmethylation reaction to drive an energy-conserving sodium ion pump
727499
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
the enzyme uses a transmethylation reaction to drive an energy-conserving sodium ion pump
727499
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
Other publictions for EC 2.1.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733227
Peterson
Towards a computational model ...
Methanosarcina acetivorans
Archaea
2014
898453
2014
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734072
Vepachedu
Role of the fused corrinoid/me ...
Methanosarcina acetivorans
J. Bacteriol.
194
4161-4168
2012
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1
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3
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1
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3
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3
1
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1
1
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687410
Welander
Mutagenesis of the C1 oxidatio ...
Methanosarcina barkeri
J. Bacteriol.
190
1928-1936
2008
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1
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2
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727499
Lienard
Cloning, sequencing and expres ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS Lett.
425
204-208
1998
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1
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1
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6
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727407
Lienard
Sodium ion translocation by N5 ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
Eur. J. Biochem.
239
857-864
1996
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1
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1
1
7
2
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3
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1
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1
1
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8
1
1
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1
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1
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1
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1
1
7
2
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1
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1
1
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8
1
1
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1
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639489
Sauer
Tetrahydromethanopterin methyl ...
Methanothermobacter thermautotrophicus
Biochem. Biophys. Res. Commun.
136
542-547
1986
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