BRENDA - Enzyme Database
show all sequences of 2.1.1.86

Tetrahydromethanopterin methyltransferase, a component of the methane synthesizing complex of Methanobacterium thermoautotrophicum

Sauer, F.D.; Biochem. Biophys. Res. Commun. 136, 542-547 (1986)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.88
-
5,6,7,8-tetrahydromethanopterin
-
Methanothermobacter thermautotrophicus
3.7
-
(2-methylthio)ethanesulfonate
-
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanothermobacter thermautotrophicus
involved in the formation of methane from CO
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
Methanothermobacter thermautotrophicus
r
Organism
Organism
UniProt
Commentary
Textmining
Methanothermobacter thermautotrophicus
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxygen-sensitive
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
r
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
involved in the formation of methane from CO
639489
Methanothermobacter thermautotrophicus
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Methanothermobacter thermautotrophicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
5 min, inactivation
Methanothermobacter thermautotrophicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
-
Methanothermobacter thermautotrophicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7
sharp decrease in activity below pH 6.0 and above pH 7.0
Methanothermobacter thermautotrophicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.88
-
5,6,7,8-tetrahydromethanopterin
-
Methanothermobacter thermautotrophicus
3.7
-
(2-methylthio)ethanesulfonate
-
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanothermobacter thermautotrophicus
involved in the formation of methane from CO
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
Methanothermobacter thermautotrophicus
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen-sensitive
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
r
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
involved in the formation of methane from CO
639489
Methanothermobacter thermautotrophicus
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
639489
Methanothermobacter thermautotrophicus
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Methanothermobacter thermautotrophicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
5 min, inactivation
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.7
-
-
Methanothermobacter thermautotrophicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7
sharp decrease in activity below pH 6.0 and above pH 7.0
Methanothermobacter thermautotrophicus
Other publictions for EC 2.1.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733227
Peterson
Towards a computational model ...
Methanosarcina acetivorans
Archaea
2014
898453
2014
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2
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1
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2
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2
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2
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2
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734072
Vepachedu
Role of the fused corrinoid/me ...
Methanosarcina acetivorans
J. Bacteriol.
194
4161-4168
2012
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1
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2
3
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1
2
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2
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3
1
9
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1
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2
3
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1
2
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3
1
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1
1
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687410
Welander
Mutagenesis of the C1 oxidatio ...
Methanosarcina barkeri
J. Bacteriol.
190
1928-1936
2008
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1
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1
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727499
Lienard
Cloning, sequencing and expres ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS Lett.
425
204-208
1998
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1
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1
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5
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2
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1
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727407
Lienard
Sodium ion translocation by N5 ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
Eur. J. Biochem.
239
857-864
1996
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1
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1
1
7
2
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2
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1
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1
1
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8
1
2
1
-
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1
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1
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1
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1
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1
1
7
2
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1
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1
1
-
8
1
1
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1
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-
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-
-
-
-
-
639489
Sauer
Tetrahydromethanopterin methyl ...
Methanothermobacter thermautotrophicus
Biochem. Biophys. Res. Commun.
136
542-547
1986
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2
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1
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2
1
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2
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1
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1
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1
1
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2
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1
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2
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1
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1
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1
1
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