Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.82 extracted from

  • Khouri, H.E.; De Luca, V.; Ibrahim, R.K.
    Enzymic synthesis of polymethylated flavonols in Chrysosplenium americanum. III. Purification and kinetic analysis of S-adenosyl-L-methionine:3-methylquercetin 7-O-methyltransferase (1988), Arch. Biochem. Biophys., 265, 1-7.
    View publication on PubMed
Search for more literature for 2.1.1.82

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 20-50% inhibition Chrysosplenium americanum
Co2+ 1 mM, 20-50% inhibition Chrysosplenium americanum
Cu2+ 1 mM, 20-50% inhibition Chrysosplenium americanum
N-ethylmaleimide
-
 Chrysosplenium americanum
p-chloromercuribenzoate
-
 Chrysosplenium americanum
Zn2+ 1 mM, 20-50% inhibition Chrysosplenium americanum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 3',4',5,7-tetrahydroxy-3-methoxyflavone
-
 Chrysosplenium americanum
0.065
-
 S-adenosyl-L-methionine
-
 Chrysosplenium americanum

Organism

Organism UniProt Comment Textmining
Chrysosplenium americanum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, gel filtration, chromatofocusing, ion exchange Chrysosplenium americanum

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxy-3-methoxyflavone = S-adenosyl-L-homocysteine + 5,3',4'-trihydroxy-3,7-dimethoxyflavone ordered bi bi mechanism, S-adenosyl-L-methionine binds first, S-adenosyl-L-homocysteine is the last product released Chrysosplenium americanum
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf shoot tip Chrysosplenium americanum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
-
 Chrysosplenium americanum

Storage Stability

Storage Stability Organism
-20°C, 200 mM Tris-HCl, pH 8.0, 10 mM dithiothreitol, 2 months, no loss of activity Chrysosplenium americanum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone trivial name 3-methylquercetin, specific for 3-methylquercetin Chrysosplenium americanum S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone
-
 ?