Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.8 extracted from

  • Tongsook, C.; Niederhauser, J.; Kronegger, E.; Straganz, G.; Macheroux, P.
    Leucine 208 in human histamine N-methyltransferase emerges as a hotspot for protein stability rationalizing the role of the L208P variant in intellectual disability (2017), Biochim. Biophys. Acta, 1863, 188-199 .
    View publication on PubMed
Search for more literature for 2.1.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
L208D mutant is severely compromised in its stability Homo sapiens
L208F 21% of wild-type activtiy, Michaelis-Menten parameters for SAM and histamine similar to wild-type Homo sapiens
L208H mutant is severely compromised in its stability Homo sapiens
L208K substantial loss of enzymatic activity and binding affinity for histamine Homo sapiens
L208N mutant is severely compromised in its stability Homo sapiens
L208R substantial loss of enzymatic activity and binding affinity for histamine Homo sapiens
L208T mutant is severely compromised in its stability Homo sapiens
L208V 48% of wild-type activity, Michaelis-Menten parameters for SAM and histamine similar to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 histamine mutant L208V, pH 8.0, 25°C Homo sapiens
0.0019
-
 histamine wild-type, pH 8.0, 25°C Homo sapiens
0.0034
-
 histamine mutant L208F, pH 8.0, 25°C Homo sapiens
0.0041
-
 S-adenosyl-L-methionine wild-type, pH 8.0, 25°C Homo sapiens
0.0068
-
 S-adenosyl-L-methionine mutant L208V, pH 8.0, 25°C Homo sapiens
0.0124
-
 S-adenosyl-L-methionine mutant L208F, pH 8.0, 25°C Homo sapiens
0.015
-
 S-adenosyl-L-methionine mutant L208K, pH 8.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P50135
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + histamine
-
 Homo sapiens S-adenosyl-L-homocysteine + Ntau-methylhistamine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.4
-
 melting temperature, mutant L208R Homo sapiens
53.7
-
 melting temperature, mutant L208K Homo sapiens
57.8
-
 melting temperature, mutant L208V Homo sapiens
60.4
-
 melting temperature, wild-type, presence of histamine, and melting temeprature of mutant L208F Homo sapiens
61.3
-
 melting temperature, wild-type Homo sapiens
61.5
-
 melting temperature, wild-type, presence of S-adenosyl-L-methionine Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 S-adenosyl-L-methionine mutant L208K, pH 8.0, 25°C Homo sapiens
0.11
-
 S-adenosyl-L-methionine mutant L208F, pH 8.0, 25°C Homo sapiens
0.23
-
 S-adenosyl-L-methionine mutant L208V, pH 8.0, 25°C Homo sapiens
0.61
-
 S-adenosyl-L-methionine wild-type, pH 8.0, 25°C Homo sapiens