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Literature summary for 2.1.1.79 extracted from
- Cyclopropane fatty acid synthase from Oenococcus oeni: expression in Lactococcus lactis subsp. cremoris and biochemical characterization (2015), Arch. Microbiol., 197, 1063-1074.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Lactococcus lactis subsp. cremoris strain MG1363 and Escherichia coli BL21(DE3) cells | Oenococcus oeni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
S-adenosyl-L-methionine | at pH 5.6 and 35°C | Oenococcus oeni |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Oenococcus oeni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + phospholipid olefinic fatty acid | Oenococcus oeni | - |
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oenococcus oeni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Oenococcus oeni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Oenococcus oeni | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Oenococcus oeni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CFA synthase | - |
Oenococcus oeni |
| cyclopropane fatty acid synthase | - |
Oenococcus oeni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Oenococcus oeni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 100 | - |
S-adenosyl-L-methionine | at pH 5.6 and 35°C | Oenococcus oeni | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Oenococcus oeni |
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Release 2023.1 (January 2023)
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