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Literature summary for 2.1.1.77 extracted from

  • Petla, B.P.; Kamble, N.U.; Kumar, M.; Verma, P.; Ghosh, S.; Singh, A.; Rao, V.; Salvi, P.; Kaur, H.; Saxena, S.C.; Majee, M.
    Rice protein L-isoaspartyl methyltransferase isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity (2016), New Phytol., 211, 627-645 .
    View publication on PubMed
Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 S-adenosyl-L-methionine isoform PIMT1, at pH 8.0 and 45°C Oryza sativa
0.01
-
 S-adenosyl-L-methionine mutant DELTAPIMT2, at pH 8.0 and 45°C Oryza sativa
0.011
-
 S-adenosyl-L-methionine isoform PIMT1, at pH 8.0 and 50°C Oryza sativa
0.133
-
 VYP-(L-iso-Asp)-HA isoform PIMT1, at pH 8.0 and 50°C Oryza sativa
0.145
-
 VYP-(L-iso-Asp)-HA mutant DELTAPIMT2, at pH 8.0 and 45°C Oryza sativa
0.153
-
 VYP-(L-iso-Asp)-HA isoform PIMT1, at pH 8.0 and 45°C Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Oryza sativa
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cultivar pusa basmati-1
-

Purification (Commentary)

Purification (Comment) Organism
nickel charged affinity column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Oryza sativa
-
endosperm
-
 Oryza sativa
-
seed
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Oryza sativa S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
S-adenosyl-L-methionine + VYP-(L-iso-Asp)-HA
-
 Oryza sativa S-adenosyl-L-homocysteine + VYP-(L-iso-Asp-alpha-methyl ester)-HA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, isoform PIMT1, SDS-PAGE Oryza sativa
monomer 1 * 31000, isoform PIMT2, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
PIMT
-
 Oryza sativa
PIMT1 isoform Oryza sativa
PIMT2 isoform Oryza sativa
protein L-isoaspartyl O-methyltransferase
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 isoform PIMT1 Oryza sativa
50
-
 isoform PIMT1 Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 isoforms PIMT1 and PIMT2 show high activity between 30 and 55°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 isoforms PIMT1 and PIMT2 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5 9 isoforms PIMT1 and PIMT2 show high activity between pH 5.0 and 9.0 Oryza sativa

Expression

Organism Comment Expression
Oryza sativa enzyme activity gradually increases as seed development proceeds, with maximum activity during maturation drying. The enzyme activity is also stimulated in the presence of various phytohormones, especially abscisic acid up

General Information

General Information Comment Organism
physiological function the enzyme repairs antioxidative enzymes and proteins which restrict reactive oxygen species accumulation, lipid peroxidation, etc. in seed, particularly during aging, thus contributing to seed vigor and longevity Oryza sativa