Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, from 25% v/v PEG 4000, 100 mM NaCl and sodium citrate buffer, pH 5.0, at 291 K using the hanging-drop vapor-diffusion method in 0.0016 ml with 8 mg/ml protein, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 | Thermotoga maritima | TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria | tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene trmFO | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 | TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria | Thermotoga maritima | tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD | - |
? | |
N5,N10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 | - |
Thermotoga maritima | tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRMFO | - |
Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Thermotoga maritima |