Literature summary for 2.1.1.72 extracted from

Wons, E.; Mruk, I.; Kaczorowski, T.
Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates (2018), Sci. Rep., 8, 8243 .

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0000244	-	double stranded DNA adenine	with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000751	-	double stranded DNA adenine	with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000782	-	double stranded DNA adenine	with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000784	-	double stranded DNA adenine	with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000855	-	double stranded DNA adenine	with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C	Escherichia coli
0.000089	-	double stranded DNA adenine	with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000912	-	double stranded DNA adenine	with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0000914	-	double stranded DNA adenine	with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C	Escherichia coli
0.0001023	-	double stranded DNA adenine	with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C	Escherichia coli
0.0001273	-	double stranded DNA adenine	with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C	Escherichia coli
0.000143	-	double stranded DNA adenine	with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C	Escherichia coli
0.0002576	-	single stranded DNA adenine	with specific sequence AAGCTT, at pH 7.0 and 37°C	Escherichia coli
0.00027	-	S-adenosyl-L-methionine	at pH 7.0 and 37°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
S-adenosyl-L-methionine + DNA adenine	Haemophilus influenzae	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	Escherichia coli	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	Lactococcus cremoris	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	Haemophilus influenzae RD	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	Lactococcus cremoris W15	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	Escherichia coli E1585-68	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli E1585-68	-	-	-
Haemophilus influenzae	-	-	-
Haemophilus influenzae RD	-	-	-
Lactococcus cremoris	-	-	-
Lactococcus cremoris W15	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT	Escherichia coli	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3')	Escherichia coli	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine	Haemophilus influenzae	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine	Lactococcus cremoris	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine	Haemophilus influenzae RD	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine	Lactococcus cremoris W15	?	-	-
additional information	the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT	Escherichia coli E1585-68	?	-	-
additional information	the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3')	Escherichia coli E1585-68	?	-	-
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Haemophilus influenzae	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Escherichia coli	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Lactococcus cremoris	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Haemophilus influenzae RD	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Lactococcus cremoris W15	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + DNA adenine	the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site	Escherichia coli E1585-68	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Haemophilus influenzae	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Escherichia coli	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Lactococcus cremoris	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Haemophilus influenzae RD	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Lactococcus cremoris W15	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + double stranded DNA adenine	-	Escherichia coli E1585-68	S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + single stranded DNA adenine	the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity	Escherichia coli	S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine	-	?
S-adenosyl-L-methionine + single stranded DNA adenine	the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity	Escherichia coli E1585-68	S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine	-	?

Synonyms

Synonyms	Comment	Organism
isospecific adenine DNA methyltransferase	-	Haemophilus influenzae
isospecific adenine DNA methyltransferase	-	Escherichia coli
isospecific adenine DNA methyltransferase	-	Lactococcus cremoris
M.EcoVIII	-	Escherichia coli
M.HindIII	-	Haemophilus influenzae
M.LlaCI	-	Lactococcus cremoris

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.0002	-	double stranded DNA adenine	with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C	Escherichia coli
0.00022	-	double stranded DNA adenine	with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C	Escherichia coli
0.00022	-	double stranded DNA adenine	with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C	Escherichia coli
0.00025	-	double stranded DNA adenine	with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C	Escherichia coli
0.00027	-	double stranded DNA adenine	with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C	Escherichia coli
0.00033	-	double stranded DNA adenine	with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C	Escherichia coli
0.00033	-	double stranded DNA adenine	with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C	Escherichia coli
0.00033	-	double stranded DNA adenine	with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.00033	-	double stranded DNA adenine	with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0005	-	single stranded DNA adenine	with specific sequence AAGCTT, at pH 7.0 and 37°C	Escherichia coli
0.0015	-	double stranded DNA adenine	with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli
0.0021	-	double stranded DNA adenine	with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
1.7	-	double stranded DNA adenine	with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C	Escherichia coli
1.7	-	double stranded DNA adenine	with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C	Escherichia coli
1.9	-	double stranded DNA adenine	with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C	Escherichia coli
1.9	-	single stranded DNA adenine	with specific sequence AAGCTT, at pH 7.0 and 37°C	Escherichia coli
2.2	-	double stranded DNA adenine	with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C	Escherichia coli
2.6	-	double stranded DNA adenine	with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C	Escherichia coli
2.7	-	double stranded DNA adenine	with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C	Escherichia coli
2.9	-	double stranded DNA adenine	with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C	Escherichia coli
4.2	-	double stranded DNA adenine	with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C	Escherichia coli
4.3	-	double stranded DNA adenine	with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C	Escherichia coli
19.6	-	double stranded DNA adenine	with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli
86.1	-	double stranded DNA adenine	with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C	Escherichia coli