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Literature summary for 2.1.1.72 extracted from

  • Gupta, Y.K.; Chan, S.H.; Xu, S.Y.; Aggarwal, A.K.
    Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I (2015), Nat. Commun., 6, 7363 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HMS174 (DE3) and ER2566 cells Caulobacter vibrioides
expressed in Escherichia coli NEB cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with duplex DNA and AMP, hanging drop vapor diffusion method, using 10% (w/v) PEG 5000 monomethyl ether, 0.1 M HEPES pH 7.5, 0.2M potassium acetate and 15 mM MnCl2 at 20°C Escherichia coli

Protein Variants

Protein Variants Comment Organism
D347A the mutant shows wild type-like methylation activity Caulobacter vibrioides
H317A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
H336A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
K267A the mutant shows wild type-like methylation activity Caulobacter vibrioides
K318A the mutant shows wild type-like methylation activity Caulobacter vibrioides
N330A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
Q324A the mutant shows wild type-like methylation activity Caulobacter vibrioides
R268A the mutant shows wild type-like methylation activity Caulobacter vibrioides
R272A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
R302A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
R350A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
R354A the mutant shows wild type-like methylation activity Caulobacter vibrioides
S315A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine in DNA Escherichia coli the enzyme specifically binds double-stranded DNA S-adenosyl-L-homocysteine + DNA containing N6-methyladenine
-
 ?
S-adenosyl-L-methionine + mRNA Caulobacter vibrioides
-
 S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ceramic hydroxylapatite column chromatography and cation exchange column chromatography Escherichia coli
Ni-NTA column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ?
-
-
S-adenosyl-L-methionine + adenine in DNA the enzyme specifically binds double-stranded DNA Escherichia coli S-adenosyl-L-homocysteine + DNA containing N6-methyladenine
-
 ?
S-adenosyl-L-methionine + mRNA
-
 Caulobacter vibrioides S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
CcrM
-
 Caulobacter vibrioides
DNA MTase
-
 Caulobacter vibrioides
DNA-(adenine N6)-methyltransferase
-
 Caulobacter vibrioides
EcoP15I
-
 Escherichia coli