Literature summary for 2.1.1.72 extracted from

Horton, J.R.; Woodcock, C.B.; Opot, S.B.; Reich, N.O.; Zhang, X.; Cheng, X.
The cell cycle-regulated DNA adenine methyltransferase CcrM opens a bubble at its DNA recognition site (2019), Nat. Commun., 10, 4600 .

Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Caulobacter vibrioides

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with double-stranded DNA containing the recognition sequence GANTC, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.8, with 10% (w/v) polyethylene glycol 8000 and 3% (w/v) polyethylene glycol 6000	Caulobacter vibrioides

Protein Variants

Protein Variants	Comment	Organism
S315A/H317A/N330A/R350A	the mutant shows about 90% reduced methylation activity on double stranded DNA but no effect on S-adenosyl-L-methionione binding	Caulobacter vibrioides
W332A	inactive	Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + DNA adenine	Caulobacter vibrioides	the enzyme methylates the adenine of hemimethylated GANTC after replication	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Caulobacter vibrioides	P0CAW2	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap nickel affinity column chromatography	Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + DNA adenine	the enzyme methylates the adenine of hemimethylated GANTC after replication	Caulobacter vibrioides	S-adenosyl-L-homocysteine + DNA 6-methyladenine	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Caulobacter vibrioides

Synonyms

Synonyms	Comment	Organism
CcrM	-	Caulobacter vibrioides
cell cycle-regulated DNA methyltransferase	-	Caulobacter vibrioides
DNA adenine methyltransferase	-	Caulobacter vibrioides

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Release 2023.1 (January 2023)