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Literature summary for 2.1.1.72 extracted from
- Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: Potential implications for methylation-independent transcriptional repression (2015), Nucleic Acids Res., 43, 4296-4308.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Hms174(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with non-cognate DNA, lacking any GATC sequences, hanging drop vapor diffusion method, using 5-40% (w/v) PEG 200 from and 100 mM MES or HEPES (pH 6.4-7.0) | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | Escherichia coli | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AEE8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity column chromatography, UnoS column chromatography, and S75 Sepharose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | relative to GATC, three near-cognate substrates that carry a base-pair substitution at the first position are still methylated by the enzyme, although at rates reduced by 100fold (AATC) or 1000fold (CATC) | Escherichia coli | ? | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | - |
Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dam | - |
Escherichia coli |
| DNA adenine methyltransferase | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme, in addition to being responsible for GATC methylation, can also function as a methylation-independent transcriptional repressor | Escherichia coli |
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Release 2023.1 (January 2023)
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