Protein Variants | Comment | Organism |
---|---|---|
D347A | the mutant shows wild type-like methylation activity | Caulobacter vibrioides |
H317A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
H336A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
K318A | the mutant shows wild type-like methylation activity | Caulobacter vibrioides |
N330A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
Q324A | the mutant shows wild type-like methylation activity | Caulobacter vibrioides |
R272A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
R302A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
R350A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
R354A | the mutant shows wild type-like methylation activity | Caulobacter vibrioides |
S315A | the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) | Caulobacter vibrioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Caulobacter vibrioides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Caulobacter vibrioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme methylates GANTC sequences but does not specifically bind to DNA containing GANTC sequences | Caulobacter vibrioides | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | a biotinylated double stranded 23mer oligodeoxynucleotide with one hemimethylated GANTC site is used as substrate | Caulobacter vibrioides | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Caulobacter vibrioides |
Synonyms | Comment | Organism |
---|---|---|
CcrM | - |
Caulobacter vibrioides |
DNA-(adenine N6)-methyltransferase | - |
Caulobacter vibrioides |