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Literature summary for 2.1.1.72 extracted from
- Novel non-specific DNA adenine methyltransferases (2012), Nucleic Acids Res., 40, 2119-2130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain ER2566 | Haemophilus influenzae |
| expressed in Escherichia coli strain ER2566 | Neisseria meningitidis |
| expressed in Escherichia coli strain ER2566 | Haemophilus influenzae biotype aegyptius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D194A | the Hia5 mutant shows loss of DNA MTase activity | Haemophilus influenzae |
| D194A | the Hia5 mutant shows loss of DNA MTase activity | Neisseria meningitidis |
| D194A | the Hia5 mutant shows loss of DNA MTase activity | Haemophilus influenzae biotype aegyptius |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33600 | - |
x * 33600, SDS-PAGE | Neisseria meningitidis |
| 34000 | - |
x * 34000, SDS-PAGE | Haemophilus influenzae biotype aegyptius |
| 38000 | - |
x * 38000, SDS-PAGE | Haemophilus influenzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
| Haemophilus influenzae biotype aegyptius | G9C944 | Hia5 protein | - |
| Haemophilus influenzae biotype aegyptius ATCC 11116 | G9C944 | Hia5 protein | - |
| Haemophilus influenzae Rd (FluMu) | - |
- |
- |
| Neisseria meningitidis | - |
- |
- |
| Neisseria meningitidis Z2491 (Pnme1) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Haemophilus influenzae |
| Ni-NTA agarose column chromatography | Neisseria meningitidis |
| Ni-NTA agarose column chromatography | Haemophilus influenzae biotype aegyptius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | - |
Neisseria meningitidis | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |  |
| S-adenosyl-L-methionine + DNA adenine | the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA | Haemophilus influenzae biotype aegyptius | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA | Haemophilus influenzae | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | - |
Neisseria meningitidis Z2491 (Pnme1) | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA | Haemophilus influenzae Rd (FluMu) | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA | Haemophilus influenzae biotype aegyptius ATCC 11116 | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Haemophilus influenzae |
| ? | x * 34000, SDS-PAGE | Haemophilus influenzae biotype aegyptius |
| ? | x * 33600, SDS-PAGE | Neisseria meningitidis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA adenine methyltransferase | - |
Haemophilus influenzae |
| DNA adenine methyltransferase | - |
Neisseria meningitidis |
| DNA adenine methyltransferase | - |
Haemophilus influenzae biotype aegyptius |
| DNA adenine N6-methyltransferase | - |
Haemophilus influenzae |
| DNA adenine N6-methyltransferase | - |
Neisseria meningitidis |
| DNA adenine N6-methyltransferase | - |
Haemophilus influenzae biotype aegyptius |
| DNAm6A MTase | - |
Haemophilus influenzae |
| DNAm6A MTase | - |
Neisseria meningitidis |
| DNAm6A MTase | - |
Haemophilus influenzae biotype aegyptius |
| Hia5 protein | - |
Haemophilus influenzae biotype aegyptius |
| Hin1523 protein | - |
Haemophilus influenzae |
| Nme1821 protein | - |
Neisseria meningitidis |
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Release 2023.1 (January 2023)
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