Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Moraxella bovis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ inhibits methylation activity | Moraxella bovis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Moraxella bovis | no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Moraxella bovis | M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella bovis | - |
strain ATCC 10900 | - |
Purification (Comment) | Organism |
---|---|
partial purification using phosphocellulose column chromatography, hydroxylapatite column chromatography, CM-Sephadex gel filtration, and heparin agarose column chromatography | Moraxella bovis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA | Moraxella bovis | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine | Moraxella bovis | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine N6-methyltransferase | - |
Moraxella bovis |
M1.MboII | a type IIS methyltransferase | Moraxella bovis |