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Literature summary for 2.1.1.72 extracted from

  • Furmanek-Blaszk, B.; Boratynski, R.; Zolcinska, N.; Sektas, M.
    M1.Mboll and M2.Mboll type IIS methyltransferases: different specificities, the same target (2009), Microbiology, 155, 1111-1121.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Moraxella bovis

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ Mg2+ inhibits methylation activity Moraxella bovis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Moraxella bovis no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA ?
-
?
S-adenosyl-L-methionine + DNA adenine Moraxella bovis M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine S-adenosyl-L-homocysteine + DNA 6-methyladenine
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?

Organism

Organism UniProt Comment Textmining
Moraxella bovis
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strain ATCC 10900
-

Purification (Commentary)

Purification (Comment) Organism
partial purification using phosphocellulose column chromatography, hydroxylapatite column chromatography, CM-Sephadex gel filtration, and heparin agarose column chromatography Moraxella bovis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA Moraxella bovis ?
-
?
S-adenosyl-L-methionine + DNA adenine M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine Moraxella bovis S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Synonyms

Synonyms Comment Organism
adenine N6-methyltransferase
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Moraxella bovis
M1.MboII a type IIS methyltransferase Moraxella bovis