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Literature summary for 2.1.1.72 extracted from
- Changing the DNA recognition specificity of the EcoDam DNA-(adenine-N6)-methyltransferase by directed evolution (2010), J. Mol. Biol., 395, 79-88.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzymes are expressed in Escherichia coli HMS174(DE3) cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R124R/P134A | the mutant shows an increase of enzyme activity at GAAC sites | Escherichia coli |
| R124S/P134A | the mutant shows an increase of enzyme activity at GATG sites | Escherichia coli |
| R124S/P134S | the mutant shows an increase of enzyme activity at GATT sites | Escherichia coli |
| R124S/P134S/K139E/F159L/K241E | the mutant shows a more than 20fold preference for methylation at GATT, overall corresponding to a 1600fold change in specificity, the mutant is virtually inactive at GATC sites | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the wild type Dam shows no detectable activity at GATT sites | ? | - |
? |  |
| S-adenosyl-L-methionine + DNA adenine | Escherichia coli | the wild type enzyme shows target specificity for GATC sites | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the wild type Dam shows no detectable activity at GATT sites | Escherichia coli | ? | - |
? | |
| S-adenosyl-L-methionine + DNA adenine | the wild type enzyme shows target specificity for GATC sites | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenine-N6 DNA methyltransferase | - |
Escherichia coli |
| Dam | - |
Escherichia coli |
| DNA-(adenine-N6)-methyltransferase | - |
Escherichia coli |
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