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Literature summary for 2.1.1.72 extracted from

  • Morgan, R.D.; Bhatia, T.K.; Lovasco, L.; Davis, T.B.
    MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection (2008), Nucleic Acids Res., 36, 6558-6570.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain ER2683 Methylophilus methylotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105100
-
calculated from amino acid sequence Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methylophilus methylotrophus MmeI does not produce any detectable N4-cytosine methylation ?
-
?
S-adenosyl-L-methionine + DNA adenine Methylophilus methylotrophus MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus B2MU09
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin Hyper D column chromatography, Source 15Q column chromatography, heparin TSK column chromatography, Source 15 S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MmeI does not produce any detectable N4-cytosine methylation Methylophilus methylotrophus ?
-
?
S-adenosyl-L-methionine + DNA adenine MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3' Methylophilus methylotrophus S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Synonyms

Synonyms Comment Organism
MmeI has both endonuclease and DNA methyltransferase activities Methylophilus methylotrophus

General Information

General Information Comment Organism
physiological function MmeI DNA methylation prevents cleavage by MmeI Methylophilus methylotrophus