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Literature summary for 2.1.1.72 extracted from

  • Thomas, C.B.; Gumport, R.I.
    Dimerization of the bacterial RsrI N6-adenine DNA methyltransferase (2006), Nucleic Acids Res., 34, 806-815.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.72

Application

Application Comment Organism
analysis the dimer stabilizes and enhances site-specific DNA-binding by the MTase, because the DNA-binding site may require an interfacial structure formed by both monomers, role of Type II MTases as defense systems against phage Cereibacter sphaeroides

Cloned(Commentary)

Cloned (Comment) Organism
mutant expressed in Escherichia coli BL21(DE3) Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
S124D reduced solubility of the protein relative to that of the wild-type enzyme, fails to crystallize under the same conditions used to crystallize wild-type enzyme, is active and shows a non-linear dependence of activity on enzyme concentration similar as wild-type Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000000000001
-
 DNA adenine His-tagged wild-type Cereibacter sphaeroides
0.00000000000002
-
 DNA adenine mutant S124D Cereibacter sphaeroides
0.00086
-
 S-adenosyl-L-methionine mutant S124D Cereibacter sphaeroides
0.0027
-
 S-adenosyl-L-methionine His-tagged wild-type Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration, mutant S124D Cereibacter sphaeroides
74400
-
 gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant purified by nickel-ion metal-affinity chromatography, more than 95% pure Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-80°C Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine
-
 Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
M.RsrI
-
 Cereibacter sphaeroides
RsrI N6-adenine DNA methyltransferase
-
 Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000167
-
 DNA adenine mutant S124D Cereibacter sphaeroides
0.0035
-
 DNA adenine His-tagged wild-type Cereibacter sphaeroides