Application | Comment | Organism |
---|---|---|
medicine | methyltransferase activity associated with Dam is essential for attenuation of Aeromonas hydrophila virulence, immunization of mice with a native-Dam-overproducing strain at the same 50% lethal dose does not result in any lethality and provides protection to animals after subsequent challenge with a lethal dose of the control strain | Aeromonas hydrophila |
Cloned (Comment) | Organism |
---|---|
native or mutated dam gene overexpressed from the pBAD/damAhSSU pBAD/damD/A, or pBAD/damY/A plasmid under the control of an araBAD promoter, transformed to Escherichia coli GM33 | Aeromonas hydrophila |
Protein Variants | Comment | Organism |
---|---|---|
D71A | phenotype of native Dam | Aeromonas hydrophila |
E76A | phenotype of native Dam | Aeromonas hydrophila |
additional information | mutated dam gene, in which either the aspartic acid or the tyrosine residue was changed to alanine, do not possess MTase activity in the DPPY motif, resistant to DpnI digestion and sensitive to DpnII restriction endonuclease cutting, overproduction in Aeromonas hydrophila results in bacterial motility, hemolytic and cytotoxic activities associated with the cytotoxic enterotoxin, and protease activity similar to that of the wild-type | Aeromonas hydrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila | - |
- |
- |
Aeromonas hydrophila SSU | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | - |
Aeromonas hydrophila | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Aeromonas hydrophila SSU | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dam | - |
Aeromonas hydrophila |
DNA adenine methyltransferase | - |
Aeromonas hydrophila |