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Literature summary for 2.1.1.72 extracted from

  • Thomas, C.B.; Scavetta, R.D.; Gumport, R.I.; Churchill, M.E.
    Structures of liganded and unliganded RsrI N6-adenine DNA methyltransferase: a distinct orientation for active cofactor binding (2003), J. Biol. Chem., 278, 26094-26101.
    View publication on PubMed
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expression of the apo-enzyme mutant L72P as His-tagged enzyme Cereibacter sphaeroides

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with substrate S-adenosyl-L-methionine, product S-adenosyl-L-homocysteine, or inhibitor sinefungin, and L72P mutant apo-enzyme, 2.0 mg/ml protein in crystallization buffer containing 100 mM HEPES, pH 7.4, 1.5 M LiSO4, and 1-20 mM of the ligand compounds, X-ray diffraction structure determination and analysis at 2.3 A resolution Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
L72P site-directed mutagenesis, altered secondary structure, the active site is pushed away from the ligand binding site, especially by altered position of Trp84 Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
sinefungin binding structure Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Cereibacter sphaeroides
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides P14751
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine
-
 Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
S-adenosyl-L-methionine + DNA adenine substrate and product binding site structures Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Synonyms

Synonyms Comment Organism
M-RsrI
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 Cereibacter sphaeroides
N6_N4_MTase
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 Cereibacter sphaeroides
RsrI N6-adenine DNA methyltransferase
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 Cereibacter sphaeroides