Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.72 extracted from

  • Kossykh, V.G.; Schlagman, S.L.; Hattman, S.
    Comparative studies of the phage T2 and T4 DNA (N6-adenine)methyltransferases: amino acid changes that affect catalytic activity (1997), J. Bacteriol., 179, 3239-3243.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning of T2 dam gene into expression vector pJW2, creation of T2-T4 hybrid genes in expression vector pJW2 Escherichia phage T2

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is inhibited at ionic strength greater than 0.2 M Escherichia phage T2
additional information
-
Tequatrovirus T4
S-adenosyl-L-homocysteine
-
Escherichia phage T2
S-adenosyl-L-homocysteine
-
Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000000011
-
adenine in T4 gt- dam- DNA nonglucosylated unmethylated T4 gt- dam- DNA Tequatrovirus T4
0.0000000011
-
adenine in T4 gt- dam- DNA nonglucosylated unmethylated T4 gt- dam- DNA Escherichia phage T2
0.0001
-
S-adenosyl-L-methionine
-
Tequatrovirus T4
0.0001
-
S-adenosyl-L-methionine
-
Escherichia phage T2

Organism

Organism UniProt Comment Textmining
Escherichia phage T2
-
-
-
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia phage T2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Tequatrovirus T4
additional information
-
-
Escherichia phage T2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA
-
Tequatrovirus T4 S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA
-
?
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA
-
Escherichia phage T2 S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA
-
?
S-adenosyl-L-methionine + DNA adenine nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?
S-adenosyl-L-methionine + DNA adenine nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does Escherichia phage T2 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Tequatrovirus T4
additional information
-
additional information residues at position 20 and 26 are responsible for the different turnover-number values of the two MTases bor both canonical and noncanonical sites Escherichia phage T2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
Escherichia phage T2

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0024
-
S-adenosyl-L-homocysteine
-
Tequatrovirus T4
0.0024
-
S-adenosyl-L-homocysteine
-
Escherichia phage T2