Literature summary for 2.1.1.72 extracted from

Zinoviev, V.V.; Evdokimov, A.A.; Gorbunov, Y.A.; Malygin, E.G.; Kossykh, V.G.; Hattman, S.
Phage T4 DNA [N6-adenine] methyltransferase. Kinetic studies using oligonucleotides containing native or modified recognition sites (1998), Biol. Chem., 379, 481-488.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the T4 Dam methylation reaction cannot be explained by a simple Michaelian scheme	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + DNA adenine	the enzyme catalyzes methyl group transfer from S-adenosyl-L-methionine to the N6-position of adenine in the palindromic sequence GATC. The rate-limiting step is release of product methylated DNA from the enzyme	Tequatrovirus T4	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?
S-adenosyl-L-methionine + DNA adenine	canonical 12-mer and 20-mers various defective duplexes containing some defect in the DNA-target site, e.g. the absence of an internucleotide phosphate or a nucleotide within the recognition site, or a single-stranded region	Tequatrovirus T4	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	turnover-numbers of the canonical 12-mer and 20 mers and for various defective duplexes	Tequatrovirus T4

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Release 2023.1 (January 2023)