General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification and storage | Petroselinum crispum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Petroselinum crispum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
S-adenosyl-L-methionine | - |
Petroselinum crispum | |
0.0098 | - |
xanthotoxol | - |
Petroselinum crispum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Petroselinum crispum |
67000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + xanthotoxol | Petroselinum crispum | final methylation in the biosynthesis of the furanocoumarin xanthotoxin | S-adenosyl-L-homocysteine + xanthotoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | - |
cv Hamburger Schnitt | - |
Purification (Comment) | Organism |
---|---|
- |
Petroselinum crispum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | from leaf petiole, cells treated with an elicitor from Phytophtora megasperma, no activity in untreated cells | Petroselinum crispum | - |
petiole | cell suspension culture | Petroselinum crispum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.155 | - |
- |
Petroselinum crispum |
Storage Stability | Organism |
---|---|
-20°C, stable for 20 days in 50% v/v glycerol | Petroselinum crispum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-hydroxyxanthotoxin | 0.7% of the activity with xanthotoxol | Petroselinum crispum | S-adenosyl-L-homocysteine + isopimpinellin | - |
? | |
S-adenosyl-L-methionine + 8-hydroxybergapten | 2% of the activity with xanthotoxol | Petroselinum crispum | S-adenosyl-L-homocysteine + isopimpinellin | - |
? | |
S-adenosyl-L-methionine + bergaptol | 0.5% of the activity with xanthotoxol | Petroselinum crispum | S-adenosyl-L-homocysteine + bergapten | - |
? | |
S-adenosyl-L-methionine + daphnetin | 0.6% of the activity with xanthotoxol | Petroselinum crispum | S-adenosyl-L-homocysteine + hydrangetin | - |
? | |
S-adenosyl-L-methionine + xanthotoxol | - |
Petroselinum crispum | S-adenosyl-L-homocysteine + xanthotoxin | - |
? | |
S-adenosyl-L-methionine + xanthotoxol | final methylation in the biosynthesis of the furanocoumarin xanthotoxin | Petroselinum crispum | S-adenosyl-L-homocysteine + xanthotoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Petroselinum crispum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Petroselinum crispum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
30 min, inactivation below | Petroselinum crispum |