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Literature summary for 2.1.1.7 extracted from
- Purification and properties of S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase from cell suspension cultures of Glycine max L (1988), Arch. Biochem. Biophys., 262, 445-454.
General Stability
| General Stability | Organism |
|---|---|
| no stabilization by glycerol, 10-40% v/v, or nicotinic acid | Glycine max |
| sulfhydryl group protecting agents required for stabilization during purification | Glycine max |
| total loss of activity by freezing at -20°C | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Glycine max |  |
| Mg2+ | slight | Glycine max | |
| p-hydroxymercuribenzoate | - |
Glycine max | |
| S-adenosyl-L-homocysteine | - |
Glycine max | |
| SH-blocking reagents | - |
Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
S-adenosyl-L-methionine | - |
Glycine max | |
| 0.078 | - |
nicotinate | - |
Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for divalent cations | Glycine max |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
ultracentrifugation, gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + nicotinate | Glycine max | - |
S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
soybean | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rigorous exclusion of O2 by saturating the buffer with N2 allows partial stabilization during purification | Glycine max |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | heterotrophic | Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, N2-atmosphere | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + nicotinate | - |
Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? | |
| S-adenosyl-L-methionine + nicotinate | high specificity for nicotinate, no other pyridine derivative is a suitable methyl group acceptor | Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Glycine max |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5.0: about 50% of maximum activity, pH 9.0: nearly inactive | Glycine max |
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