Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Peucedanum praeruptorum |
Protein Variants | Comment | Organism |
---|---|---|
D226A | the mutant is almost inactive | Peucedanum praeruptorum |
F171A | the mutant is almost inactive | Peucedanum praeruptorum |
G203A | the mutant shows about 75% of wild type activity | Peucedanum praeruptorum |
H264A | the mutant is almost inactive | Peucedanum praeruptorum |
L312A | the mutant is almost inactive | Peucedanum praeruptorum |
M175A | the mutant is almost inactive | Peucedanum praeruptorum |
M316A | the mutant shows about 70% of wild type activity | Peucedanum praeruptorum |
Y319A | the mutant shows about 60% of wild type activity | Peucedanum praeruptorum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Peucedanum praeruptorum | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + bergaptol | Peucedanum praeruptorum | the enzyme has rigid substrate affinity to bergaptol | S-adenosyl-L-homocysteine + bergapten | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peucedanum praeruptorum | A0A166U5H3 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA column chromatography | Peucedanum praeruptorum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | lowest expression | Peucedanum praeruptorum | - |
root | high expression | Peucedanum praeruptorum | - |
stem | low expression | Peucedanum praeruptorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has no activity with xanthotol, (E)-2'-hydroxychalcone, (E)-3'-hydroxychalcone, caffeic acid, 4-hydroxycoumarin, 7-hydroxycoumarin,4,7-dihydroxycoumarin, 5,7-dihydroxyflavone, ferulic acid, and resveratrol | Peucedanum praeruptorum | ? | - |
- |
|
S-adenosyl-L-methionine + bergaptol | the enzyme has rigid substrate affinity to bergaptol | Peucedanum praeruptorum | S-adenosyl-L-homocysteine + bergapten | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, calculated from amino acid sequence | Peucedanum praeruptorum |
Synonyms | Comment | Organism |
---|---|---|
bergaptol O-methyltransferase | - |
Peucedanum praeruptorum |
BMT | - |
Peucedanum praeruptorum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Peucedanum praeruptorum | calculated from amino acid sequence and estimated by SDS-PAGE | - |
5.6 |
Organism | Comment | Expression |
---|---|---|
Peucedanum praeruptorum | the enzyme expression can be induced by methyl jasmonate | up |