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Literature summary for 2.1.1.69 extracted from

  • Zhao, Y.; Wang, N.; Zeng, Z.; Xu, S.; Huang, C.; Wang, W.; Liu, T.; Luo, J.; Kong, L.
    Cloning, functional characterization, and catalytic mechanism of a bergaptol O-methyltransferase from Peucedanum praeruptorum Dunn (2016), Front. Plant Sci., 7, 722 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Peucedanum praeruptorum

Protein Variants

Protein Variants Comment Organism
D226A the mutant is almost inactive Peucedanum praeruptorum
F171A the mutant is almost inactive Peucedanum praeruptorum
G203A the mutant shows about 75% of wild type activity Peucedanum praeruptorum
H264A the mutant is almost inactive Peucedanum praeruptorum
L312A the mutant is almost inactive Peucedanum praeruptorum
M175A the mutant is almost inactive Peucedanum praeruptorum
M316A the mutant shows about 70% of wild type activity Peucedanum praeruptorum
Y319A the mutant shows about 60% of wild type activity Peucedanum praeruptorum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Peucedanum praeruptorum 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + bergaptol Peucedanum praeruptorum the enzyme has rigid substrate affinity to bergaptol S-adenosyl-L-homocysteine + bergapten
-
 ?

Organism

Organism UniProt Comment Textmining
Peucedanum praeruptorum A0A166U5H3
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA column chromatography Peucedanum praeruptorum

Source Tissue

Source Tissue Comment Organism Textmining
leaf lowest expression Peucedanum praeruptorum
-
root high expression Peucedanum praeruptorum
-
stem low expression Peucedanum praeruptorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has no activity with xanthotol, (E)-2'-hydroxychalcone, (E)-3'-hydroxychalcone, caffeic acid, 4-hydroxycoumarin, 7-hydroxycoumarin,4,7-dihydroxycoumarin, 5,7-dihydroxyflavone, ferulic acid, and resveratrol Peucedanum praeruptorum ?
-
-
S-adenosyl-L-methionine + bergaptol the enzyme has rigid substrate affinity to bergaptol Peucedanum praeruptorum S-adenosyl-L-homocysteine + bergapten
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, calculated from amino acid sequence Peucedanum praeruptorum

Synonyms

Synonyms Comment Organism
bergaptol O-methyltransferase
-
 Peucedanum praeruptorum
BMT
-
 Peucedanum praeruptorum

pI Value

Organism Comment pI Value Maximum pI Value
Peucedanum praeruptorum calculated from amino acid sequence and estimated by SDS-PAGE
-
 5.6

Expression

Organism Comment Expression
Peucedanum praeruptorum the enzyme expression can be induced by methyl jasmonate up