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Literature summary for 2.1.1.69 extracted from

  • Lo, S.; Chung, P.; Wang, C.
    Molecular cloning and functional analysis of bergaptol-O-methyltransferase from Angelica dahurica (Bai Zhi) and using it to efficiently produce bergapten in E. coli (2012), Bot. Stud., 53, 197-206.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme is expressed in Escherichia coli Angelica dahurica

Inhibitors

Inhibitors Comment Organism Structure
Co2+ severe inhibition at 1.5 mM Angelica dahurica
Cu2+ severe inhibition at 0.1 mM, complete inhibition at 1.5 mM Angelica dahurica
additional information Fe2+ and Zn2+ only slightly affect enzyme activity at 0.1 mM. The addition of Mn2+, Fe3+, and Ca2+ barely affects enzyme catalytic activity. The enzyme is not inhibited by EDTA Angelica dahurica
Ni2+ severe inhibition at 0.1 mM, complete inhibition at 1.5 mM Angelica dahurica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
bergaptol recombinant enzyme, at pH 7.5, at 35°C Angelica dahurica
10.68
-
S-adenosyl-L-methionine recombinant enzyme, at pH 7.5, at 35°C Angelica dahurica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require a divalent cation for activity Angelica dahurica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, calculated from amino acid sequence and estimated from SDS-PAGE Angelica dahurica

Organism

Organism UniProt Comment Textmining
Angelica dahurica J9PHM5 Bai Zhi
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulfate precipitation Angelica dahurica

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Angelica dahurica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + bergaptol
-
Angelica dahurica S-adenosyl-L-homocysteine + bergapten
-
?

Subunits

Subunits Comment Organism
? x * 39000, calculated from amino acid sequence and estimated from SDS-PAGE Angelica dahurica

Synonyms

Synonyms Comment Organism
bergaptol-5-O-methyltransferase
-
Angelica dahurica
BMT
-
Angelica dahurica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Angelica dahurica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 42
-
Angelica dahurica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
in potassium phosphate buffer Angelica dahurica

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
Angelica dahurica

pI Value

Organism Comment pI Value Maximum pI Value
Angelica dahurica calculated from amino acid sequence
-
5.9