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Literature summary for 2.1.1.68 extracted from

  • Collendavelloo, J.; Legrand, M.; Geoffroy, P.; Barthelemy, J.; Fritig, B.
    Purification and properties of the three o-diphenol-O-methyltransferases of tobacco leaves (1981), Phytochemistry, 20, 611-616.
No PubMed abstract available

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Nicotiana tabacum
freezing causes complete inactivation Nicotiana tabacum
gel filtration leads to severe loss of activity Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
S-adenosyl-L-methionine
-
Nicotiana tabacum
0.03
-
esculetin isozyme I, m- and p-methylation Nicotiana tabacum
0.04
-
homocatechol isozyme I Nicotiana tabacum
0.065
-
5-hydroxyferulic acid
-
Nicotiana tabacum
0.1
-
caffeic acid isozyme I Nicotiana tabacum
0.23
-
Hydrocaffeic acid isozyme I Nicotiana tabacum
0.7
-
Protocatechuic aldehyde isozyme I, m- and p-methylation Nicotiana tabacum
2
-
catechol isozyme I Nicotiana tabacum
2.5
-
chlorogenic acid isozyme I Nicotiana tabacum
2.5
-
protocatechuic acid isozyme I, m- and p-methylation Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no Mg2+ requirement Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
isozyme I, gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate Nicotiana tabacum i.e. caffeic acid, involved in the biosynthesis of lignin S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
tobacco, strain Samsun NN, three isozymes isozyme I, isozyme II and isozyme III, only isozyme I has the characteristics of caffeate O-methyltransferase
-

Purification (Commentary)

Purification (Comment) Organism
three caffeic acid methylating activities: isozymes I, II, III, with an enzyme density of 1.277 kg/l, determined by isopycnic equilibrium centrifugation Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf highly infected by tobacco mosaic virus Nicotiana tabacum
-

Storage Stability

Storage Stability Organism
0°C, 65%-85% of initial activity retained after 5 months in the presence of 2-mercaptoethanol Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information p- and m-methylation of protocatechuic aldehyde and acid, as well as esculetin Nicotiana tabacum ?
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
-
Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeate, m-specific methylation Nicotiana tabacum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeic acid, involved in the biosynthesis of lignin Nicotiana tabacum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
?
S-adenosyl-L-methionine + 3-(3,4-dihydroxyphenyl)propanoic acid
-
Nicotiana tabacum S-adenosyl-L-homocysteine + 3-(4-hydroxy-3-methoxyphenyl)propanoic acid
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid best substrate Nicotiana tabacum S-adenosyl-L-homocysteine + sinapic acid
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid m-specific methylation Nicotiana tabacum S-adenosyl-L-homocysteine + sinapic acid
-
?
S-adenosyl-L-methionine + catechol isozyme II and III, no substrate for isozyme I Nicotiana tabacum S-adenosyl-L-homocysteine + 2-methoxyphenol
-
?
S-adenosyl-L-methionine + chlorogenic acid
-
Nicotiana tabacum ?
-
?
S-adenosyl-L-methionine + esculetin
-
Nicotiana tabacum ?
-
?
S-adenosyl-L-methionine + homocatechol
-
Nicotiana tabacum S-adenosyl-L-homocysteine + 2-methoxy-4-methylphenol
-
?
S-adenosyl-L-methionine + protocatechuic acid
-
Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
?
S-adenosyl-L-methionine + protocatechuic aldehyde
-
Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
isozyme I inactivates after 5 min, isozyme II loses 70% of activity after 2 h, isozyme III has 50% activation after 2 h Nicotiana tabacum