Literature summary for 2.1.1.67 extracted from

Wennerstrand, P.; Blissing, A.; Martensson, L.G.
In vitro protein stability of two naturally occurring thiopurine S-methyltransferase variants biophysical characterization of TPMT*6 and TPMT*8 (2017), ACS Omega, 2, 4991-4999 .

Search for more literature for 2.1.1.67

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R215H	the mutant shows intermediate in vivo activity compared to the wild type enzyme	Homo sapiens
Y180F	the mutant shows low in vivo activity compared to the wild type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P51580	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 6-mercaptopurine	-	Homo sapiens	S-adenosyl-L-homocysteine + 6-methylmercaptopurine	-	?

Synonyms

Synonyms	Comment	Organism
TPMT	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
46.4	-	melting temperature of the wild type enzyme without ligand	Homo sapiens
46.8	-	melting temperature of the wild type enzyme in complex with S-adenosyl-L-methionine	Homo sapiens

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Release 2023.1 (January 2023)