BRENDA - Enzyme Database show
show all sequences of 2.1.1.65

Enzymic O-methylation of isoliquiritigenin and licodione in alfalfa and licorice cultures

Ichimura, M.; Furuno, T.; Takahashi, T.; Dixon, R.A.; Ayabe, S.; Phytochemistry 44, 991-995 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
treatment with yeast extracts increases activity with the maximum at 16 to 24 h
Glycyrrhiza echinata
additional information
treatment with yeast extracts increases activity with the maximum at 24 h
Medicago sativa
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Medicago sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + licodione
Medicago sativa
biosynthesis pathway
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
?
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
biosynthesis pathway
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
Medicago sativa
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
?
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza echinata
-
-
-
Medicago sativa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycyrrhiza echinata
-
cell culture
-
Medicago sativa
-
root
-
Medicago sativa
-
shoot
-
Medicago sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
biosynthesis pathway
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
biosynthesis pathway
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Glycyrrhiza echinata
8
-
-
Medicago sativa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
treatment with yeast extracts increases activity with the maximum at 16 to 24 h
Glycyrrhiza echinata
additional information
treatment with yeast extracts increases activity with the maximum at 24 h
Medicago sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Medicago sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + licodione
Medicago sativa
biosynthesis pathway
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
?
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
biosynthesis pathway
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
Medicago sativa
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
?
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycyrrhiza echinata
-
cell culture
-
Medicago sativa
-
root
-
Medicago sativa
-
shoot
-
Medicago sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
biosynthesis pathway
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
biosynthesis pathway
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485511
Medicago sativa
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
-
-
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485511
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485511
Glycyrrhiza echinata
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Glycyrrhiza echinata
8
-
-
Medicago sativa
Other publictions for EC 2.1.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485511
Ichimura
Enzymic O-methylation of isoli ...
Glycyrrhiza echinata, Medicago sativa
Phytochemistry
44
991-995
1997
2
-
1
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
4
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
485509
Ayabe
Regulation of retrochalcone bi ...
Glycyrrhiza echinata
Plant Cell Rep.
6
16-19
1987
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485510
Ayabe
-
Biosynthesis of a retrochalcon ...
Glycyrrhiza echinata
Phytochemistry
19
2331-2336
1980
1
-
-
-
-
-
2
2
-
1
-
1
-
1
-
-
1
-
-
1
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
1
-
1
-
-
-
1
-
1
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-