BRENDA - Enzyme Database show
show all sequences of 2.1.1.65

Biosynthesis of a retrochalcone echinatin: involvement of o-methyltransferase to licodione

Ayabe, S.; Yoshikawa, T.; Kobayashi, M.; Furuya, T.; Phytochemistry 19, 2331-2336 (1980)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
slight increase of activity
Glycyrrhiza echinata
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
80% loss of activity
Glycyrrhiza echinata
Zn2+
30% loss of activity
Glycyrrhiza echinata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0028
-
S-adenosyl-L-methionine
-
Glycyrrhiza echinata
0.011
-
licodione
-
Glycyrrhiza echinata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
not requirement
Glycyrrhiza echinata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza echinata
-
strains W, KN, MS, DK
-
Purification (Commentary)
Commentary
Organism
-
Glycyrrhiza echinata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycyrrhiza echinata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Glycyrrhiza echinata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 2'-deoxylicodione
very poor substrate
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 2'-hydroxylicodione
methylated at 38% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 2,4-dihydroxybenzoic acid
methylated at 4.5% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2-methoxy-4-hydroxybenzoic acid
-
-
-
?
S-adenosyl-L-methionine + dihydroisoliquiritigenin
methylated at 7.2% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyldihydroisoliquiritigenin
-
-
-
?
S-adenosyl-L-methionine + echinatin
methylated at 4% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + isoliquiritigenin
methylated at 10.7% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
-
-
?
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + resacetophenone
methylated at 2.6% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Glycyrrhiza echinata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Glycyrrhiza echinata
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
slight increase of activity
Glycyrrhiza echinata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
80% loss of activity
Glycyrrhiza echinata
Zn2+
30% loss of activity
Glycyrrhiza echinata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0028
-
S-adenosyl-L-methionine
-
Glycyrrhiza echinata
0.011
-
licodione
-
Glycyrrhiza echinata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
not requirement
Glycyrrhiza echinata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + licodione
Glycyrrhiza echinata
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
Glycyrrhiza echinata
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Glycyrrhiza echinata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycyrrhiza echinata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Glycyrrhiza echinata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 2'-deoxylicodione
very poor substrate
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 2'-hydroxylicodione
methylated at 38% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 2,4-dihydroxybenzoic acid
methylated at 4.5% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2-methoxy-4-hydroxybenzoic acid
-
-
-
?
S-adenosyl-L-methionine + dihydroisoliquiritigenin
methylated at 7.2% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyldihydroisoliquiritigenin
-
-
-
?
S-adenosyl-L-methionine + echinatin
methylated at 4% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + isoliquiritigenin
methylated at 10.7% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
-
-
?
S-adenosyl-L-methionine + licodione
i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
hydroxy-group ortho to C3-linkage in licodione is methylated
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + licodione
involved in retrochalcone echinatin biosynthesis, selective induction in response to stress
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
485510
Glycyrrhiza echinata
?
S-adenosyl-L-methionine + resacetophenone
methylated at 2.6% of licodione methylation
485510
Glycyrrhiza echinata
S-adenosyl-L-homocysteine + ?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Glycyrrhiza echinata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Glycyrrhiza echinata
Other publictions for EC 2.1.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485511
Ichimura
Enzymic O-methylation of isoli ...
Glycyrrhiza echinata, Medicago sativa
Phytochemistry
44
991-995
1997
2
-
1
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
4
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
485509
Ayabe
Regulation of retrochalcone bi ...
Glycyrrhiza echinata
Plant Cell Rep.
6
16-19
1987
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485510
Ayabe
-
Biosynthesis of a retrochalcon ...
Glycyrrhiza echinata
Phytochemistry
19
2331-2336
1980
1
-
-
-
-
-
2
2
-
1
-
1
-
1
-
-
1
-
-
1
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
1
-
1
-
-
-
1
-
1
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-