Cloned (Comment) | Organism |
---|---|
His6-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using either 0.1 M HEPES pH 7.5 and 20% polyethylene glycol 10000 or 10% (v/v) 2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.0 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26600 | - |
x * 26600, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, DEAE column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
? | x * 26600, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
O-methyltransferase | - |
Escherichia coli |
UbiG | - |
Escherichia coli |