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Literature summary for 2.1.1.63 extracted from

  • Perugino, G.; Miggiano, R.; Serpe, M.; Vettone, A.; Valenti, A.; Lahiri, S.; Rossi, F.; Rossi, M.; Rizzi, M.; Ciaramella, M.
    Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein (2015), Nucleic Acids Res., 43, 8801-8816 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ABLE-C cells Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.35 M potassium nitrate and 1.6 M ammonium sulfate Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C119F the mutant shows reduced thermal stability compared to the wild type enzyme Saccharolobus solfataricus
C119L the mutant shows reduced thermal stability compared to the wild type enzyme Saccharolobus solfataricus
D27A the mutant shows reduced thermal stability compared to the wild type enzyme Saccharolobus solfataricus
D27K the mutant shows reduced thermal stability compared to the wild type enzyme Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Saccharolobus solfataricus
-
 DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Saccharolobus solfataricus P2
-
 DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VW7
-
-
Saccharolobus solfataricus P2 Q97VW7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
 Saccharolobus solfataricus DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
 Saccharolobus solfataricus P2 DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
alkylated DNA-protein alkyltransferase
-
 Saccharolobus solfataricus
OGT
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 melting temperature Saccharolobus solfataricus