Protein Variants | Comment | Organism |
---|---|---|
C5A | smaller increase in stability on zinc addition than the wild-type hAGT | Homo sapiens |
H29A | does not show the stability enehancement of wild-type hAGT with its intact zinc coordination sphere | Homo sapiens |
H85A | does not show the stability enehancement of wild-type hAGT with its intact zinc coordination sphere | Homo sapiens |
General Stability | Organism |
---|---|
zinc provides conformational stability to hAGT that may influence its regulation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | in bacterial expression systems, recombinant hAGT is produced in increasingly larger quantities when growth media are supplemented with up to 0.1 mM ZnCl2. Metal-enriched hAGT samples have a 5fold increase in repair rate constant over conventionally purified protein samples and a 60fold increase over metal-stripped hAGT. Zinc confers a mechanistic enhancement to repair activity that does not result from an increase in substrate binding affinity. Zinc also provides conformational stability to hAGT that may influence its regulation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
hAGT | - |
Homo sapiens |