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Literature summary for 2.1.1.61 extracted from

  • Hagervall, T.G.; Edmonds, C.G.; McCloskey, J.A.; Björk, G.R.
    Transfer RNA(5-methylaminomethyl-2-thiouridine)-methyltransferase from Escherichia coli K-12 has two enzymatic activities (1987), J. Biol. Chem., 262, 8488-8495.
    View publication on PubMed
Search for more literature for 2.1.1.61

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by putrescine or spermidine Escherichia coli
NH4+
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ strong, 5 mM Escherichia coli
additional information no inhibition by putrescine or spermidine Escherichia coli
NaCl mutant trmC1: 100 mM, mutant trmC2: 200 mM Escherichia coli
p-chloromercuribenzoate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ activation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 1 * 79000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12 strain pTH32/1100, harbouring multi-copy plasmid
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Escherichia coli
on Heparin-Sepharose Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 5-aminomehtyl-2-thiouridine34 in tRNALys = S-adenosyl-L-homocysteine + 5-methylaminomehtyl-2-thiouridine34 in tRNALys mechanismn Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1686
-
 mutant trmC1 Escherichia coli
0.9252
-
 mutant trmC2 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits two activities: enzyme from trmC1 mutant reacts with 5-carboxymethylaminomethyl-2-thiouridine to form 5-aminomethyl-2-thiouridine without S-adenosyl-L-methionine, enzyme from trmC2 mutant methylates 5-aminomethyl-2-thiouridine in the presence of S-adenosyl-L-methionine Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
-
 ?
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA tRNA from E. coli mutants Escherichia coli S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
-
 ?
S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine
-
 Escherichia coli S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 79000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.23
-
 tRNA containing 5-aminomethyl-2-thiouridine
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Escherichia coli